Description
GenElute™-E Single Spin Plant DNA 96 Kit quickly purifies genomic DNA from various species and types of plant tissues, including leaf, seed, fruit and root material. The included grinding suspension enables a fast and effective disruption of plant tissue without bead beating. The subsequent lysis step takes place under physiological conditions where the enzyme activity of proteases is generally increased. SmartLyse™ Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This eliminates the requirement for extended, overnight incubations, which are required in many conventional protocols. Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is equal or better than silica-based methods depending on sample type. GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8